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| Harney, Ewan; Dubief, Bruno; Boudry, Pierre; Basuyaux, Olivier; Schilhabel, Markus B.; Huchette, Sylvain; Paillard, Christine; Nunes, Flavia. |
| The European abalone Haliotis tuberculata is a delicacy and consequently a commercially valuable gastropod species. Aquaculture production and wild populations are subjected to multiple climate-associated stressors and anthropogenic pressures, including rising sea-surface temperatures, ocean acidification and an emerging pathogenic Vibrio infection. Transcript expression data provides a valuable resource for understanding abalone responses to variation in the biotic and abiotic environment. To generate an extensive transcriptome, we performed next-generation sequencing of RNA on larvae exposed to temperature and pH variation and on haemolymph of adults from two wild populations after experimental infection with Vibrio harveyi. We obtained more than 1.5... |
| Tipo: Text |
Palavras-chave: RNA-Seq; Mollusca; Gastropoda; Development; Vibrio harveyi; Climate change. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00318/42940/45103.pdf |
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| Harney, Ewan; Rastrick, Samuel; Artigaud, Sébastien; Pisapia, Julia; Miner, Philippe; Pichereau, Vianney; Strand, Oivind; Boudry, Pierre; Charrier, Gregory. |
| Elevated atmospheric CO2 is a major driver of global change in the ocean, causing sea surface temperatures to rise and acidification of marine environments. Together these stressors may have additive, synergistic or unforeseen consequences for many marine organisms, particularly during early life stages. The economically important King Scallop (Pecten maximus) is found along a large latitudinal gradient in the Eastern Atlantic, but it is unclear how this species will respond to changing environmental conditions. Studies of genetic structure suggest that Atlantic populations (from Spain to the UK) are genetically distinct from Norwegian populations, a result that is borne out by phenotypic differences. Yet whether genetic differences arise as a result of... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00404/51520/52114.pdf |
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| Harney, Ewan; Artigaud, Sebastien; Le Souchu, Pierrick; Miner, Philippe; Corporeau, Charlotte; Essid, Hafida; Pichereau, Vianney; Nunes, Flavia. |
| Increasing atmospheric carbon dioxide results in ocean acidification and warming, significantly impacting marine invertebrate larvae development. We investigated how ocean acidification in combination with warming affected D-veliger larvae of the Pacific oyster Crassostrea gigas. Larvae were reared for 40 h under either control (pH 8.1, 20 °C), acidified (pH 7.9, 20 °C), warm (pH 8.1, 22 °C) or warm acidified (pH 7.9, 20 °C) conditions. Larvae in acidified conditions were significantly smaller than in the control, but warm acidified conditions mitigated negative effects on size, and increased calcification. A proteomic approach employing two-dimensional electrophoresis (2-DE) was used to quantify proteins and relate their abundance to phenotypic traits. In... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Ocean acidification; Larval development; ATP synthase; GAPDH; Superoxide dismutase. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00299/40985/40076.pdf |
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| Harney, Ewan; Lachambre, Sebastien; Roussel, Sabine; Huchette, Sylvain; Enez, Florian; Morvezen, Romain; Haffray, Pierrick; Boudry, Pierre. |
| Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by... |
| Tipo: Text |
Palavras-chave: Abalone; Parentage assignment; Pedigree; Selective breeding; Transcriptome; SNP. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00429/54061/55396.pdf |
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